article : Know the procedure of Methylene Blue Stain

Methylene Blue is a homologue of Toluidine Blue O. This stain is used to visualize intracellular metachromatic granules.

Procedure:

1. Smears are fixed with heat and allowed to cool.
   
   
2. Stain with the methylene blue (homologue of toluidine blue O) solution two to seven minutes.
   
   
3. Wash slide and blot dry.

Results: By this method, the intracellular metachromatic granules stain a ruby-red to black color; with the remainder of the cell staining a pale blue color.

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Revised: August 2000
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